Quantification of 2,4-D on solid-phase exposure sampling media by LC-MS-MS

J Anal Toxicol. 2005 Apr;29(3):188-92. doi: 10.1093/jat/29.3.188.

Abstract

Three types of solid-phase chemical exposure sampling media: cellulose, polyurethane foam, and XAD-2, were analyzed for 2,4-dichlorophenoxyacetic acid (2,4-D) and the amine salts of 2,4-D. Individual samples were extracted into acidified methanol, and the extracts were analyzed via liquid chromatography-tandem mass spectrometry using electrospray ionization and a C(8) reversed-phase LC column. (13)C 2,4-D and a labeled benzoic acid (13)C or d5) were employed as the surrogate and internal standard, respectively. Over a three-year study of pesticide exposure among agricultural workers involving a high volume of analyses, values of average % recovery for (13)C 2,4-D on personal exposure samples ranged from 87 to 98 with a % relative standard deviation ranging from 7 to 12. The performance of the method compares favorably with that of the current National Institute for Occupational Safety and Health-recommended approach for the analysis of 2,4-D on these types of exposure sampling matrices.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Validation Study

MeSH terms

  • 2,4-Dichlorophenoxyacetic Acid / analysis*
  • Calibration
  • Chromatography, High Pressure Liquid
  • Herbicides / analysis*
  • Humans
  • Mass Spectrometry
  • Occupational Exposure / analysis*
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Specimen Handling

Substances

  • Herbicides
  • 2,4-Dichlorophenoxyacetic Acid