Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif

Virus Res. 2005 Jun;110(1-2):125-31. doi: 10.1016/j.virusres.2005.01.012.


The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3'-end of the intergenic hairpin, i.e. position 1568-1574 for N and position 1852-1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3'-end. The potential involvement of the 3'-end structure in transcription termination is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics*
  • Base Sequence
  • Blotting, Northern
  • Molecular Sequence Data
  • Nucleic Acid Conformation*
  • RNA, Messenger / chemistry*
  • RNA, Messenger / genetics
  • RNA, Viral / chemistry*
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tospovirus / genetics*


  • 3' Untranslated Regions
  • RNA, Messenger
  • RNA, Viral