Molecular cloning of epididymal and seminal vesicular transcripts encoding a semenogelin-related protein

Proc Natl Acad Sci U S A. 1992 May 15;89(10):4559-63. doi: 10.1073/pnas.89.10.4559.


Freshly ejaculated human semen has the appearance of a loose gel in which the predominant structural protein components are the seminal vesicle-secreted semenogelins (Sg). The primary structure of the 439-residue SgI has previously been obtained by cDNA cloning. This cDNA cross-hybridizes to a larger transcript coding for a second secretory protein, SgII. Here we report the almost complete structure of a precursor of SgII established by lambda gt11 clones isolated from epididymal and seminal vesicular cDNA libraries. The deduced amino acid sequence of the 559-residue mature protein has a molecular weight of 62,931 but an increase in weight may be provided by asparagine-linked oligosaccharide attachment at residue 249. SgII, which has 78% overall identity with SgI, contains eight 60-residue regions that display conspicuous internal sequence similarity, whereas SgI only contains six of these regions. The SgII structure is translated from an open reading frame in a polyadenylylated 2.4-kilobase transcript. The message is abundant in the seminal vesicles but rare in the epididymis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular / methods
  • DNA / genetics
  • DNA / isolation & purification
  • Epididymis / physiology*
  • Gene Library
  • Gonadal Steroid Hormones / genetics*
  • Humans
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Oligodeoxyribonucleotides
  • Protein Precursors / genetics*
  • Restriction Mapping
  • Semen / physiology*
  • Seminal Plasma Proteins*
  • Seminal Vesicle Secretory Proteins*
  • Seminal Vesicles / physiology*
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic*


  • Gonadal Steroid Hormones
  • Oligodeoxyribonucleotides
  • Protein Precursors
  • Seminal Plasma Proteins
  • Seminal Vesicle Secretory Proteins
  • seminal vesicle-specific antigen
  • DNA

Associated data

  • GENBANK/M81652