Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis

FEBS Lett. 2005 Apr 25;579(11):2451-7. doi: 10.1016/j.febslet.2005.03.047.

Abstract

Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain 1 (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-EXPB1-D2 was remarkably similar to plant expansins. Gr-EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil-inhabiting Actinobacteria, and includes a bacterial type II carbohydrate-binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Southern
  • DNA, Complementary / genetics
  • Evolution, Molecular
  • Genome
  • Helminth Proteins / chemistry*
  • Helminth Proteins / genetics
  • Helminth Proteins / metabolism*
  • Models, Molecular*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Sequence Alignment
  • Solanum tuberosum / parasitology*
  • Tobacco / genetics
  • Tylenchida / chemistry*
  • Tylenchida / genetics*

Substances

  • DNA, Complementary
  • Helminth Proteins