Heteronuclear two-bond correlation: suppressing heteronuclear three-bond or higher NMR correlations while enhancing two-bond correlations even for vanishing 2J(CH)

Nils T Nyberg; Jens O Duus; Ole W Sørensen

doi:10.1021/ja050878w

Heteronuclear two-bond correlation: suppressing heteronuclear three-bond or higher NMR correlations while enhancing two-bond correlations even for vanishing 2J(CH)

J Am Chem Soc. 2005 May 4;127(17):6154-5. doi: 10.1021/ja050878w.

Authors

Nils T Nyberg¹, Jens O Duus, Ole W Sørensen

Affiliation

¹ Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.

PMID: 15853304
DOI: 10.1021/ja050878w

Abstract

A novel two-dimensional NMR pulse sequence, H2BC, for long-range correlation of 1H and 13C nuclei is presented. The experiment has several attractive features compared to the widely used HMBC experiment, for example, (a) typically strong enhancement of correlations over two bonds while suppressing those over more bonds, that is, resolving ambiguities in standard HMBC spectra and showing two-bond correlations not present in HMBC spectra, (b) independence of long-range 1H-13C coupling constants, (c) full homo- and heteronuclear decoupling in the indirect dimension and heteronuclear decoupling in the acquisition dimension, (d) pure 2D absorption peak shapes, and (e) a pulse sequence duration significantly shorter than that of HMBC. The experiment is quite complementary to HMBC and does not effect correlations to quaternary carbons that must be obtained by HMBC.

MeSH terms

Carbon Isotopes
Nuclear Magnetic Resonance, Biomolecular / methods*
Strychnine / chemistry*

Substances

Carbon Isotopes
Strychnine