RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus

Curr Biol. 2005 Apr 26;15(8):743-9. doi: 10.1016/j.cub.2005.02.060.


The Dlk1-Gtl2 imprinted domain, encompassing the callipyge (CLPG) locus in sheep, has recently been shown to harbor a large number of maternally expressed miRNA genes [1, 2]. Two of these (mir127 and mir136) are processed from a transcript (antiPeg11) that is antisense to Rtl1/Peg11, a paternally expressed intronless gene with homology to the gag and pol polyproteins of Sushi-like retroelements [3]. We herein demonstrate that several additional miRNAs are processed from antiPeg11 and that these regulate Rtl1/Peg11 in trans by guiding RISC-mediated cleavage of its mRNA. This is the first demonstration of miRNA-mediated RNAi involving imprinted genes in mammals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Computational Biology
  • Gene Components
  • Genomic Imprinting / genetics*
  • Glycoproteins / genetics*
  • Inheritance Patterns / genetics
  • Mammals / genetics*
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Molecular Sequence Data
  • Mutation / genetics
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA Interference*
  • RNA, Messenger / metabolism*
  • Sequence Alignment


  • Glycoproteins
  • MicroRNAs
  • Proteins
  • RNA, Messenger