Substrate-dependent utilization of OprM or OpmH by the Pseudomonas aeruginosa MexJK efflux pump

Antimicrob Agents Chemother. 2005 May;49(5):2133-6. doi: 10.1128/AAC.49.5.2133-2136.2005.

Abstract

MexJK requires OprM for erythromycin efflux but not for triclosan efflux. Deletion of 15 OprM family outer membrane proteins (OMPs) revealed that only the TolC homolog OpmH functions with MexJK for triclosan efflux. This is the first report of natural utilization of multiple OMPs by a given resistance nodulation cell division transporter/membrane fusion protein pair.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / metabolism
  • Bacterial Outer Membrane Proteins / metabolism*
  • Erythromycin / metabolism
  • Membrane Proteins / metabolism
  • Membrane Transport Proteins / metabolism*
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Pseudomonas aeruginosa / enzymology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Triclosan / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Outer Membrane Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • OpmH protein, Pseudomonas aeruginosa
  • OprM protein, Pseudomonas aeruginosa
  • Triclosan
  • Erythromycin