Mice immunized with a subviral particle containing the Japanese encephalitis virus prM/M and E proteins are protected from lethal JEV infection

Virology. 1992 Jun;188(2):714-20. doi: 10.1016/0042-6822(92)90526-u.


Extracellular subviral particles produced by HeLa cells infected with a recombinant vaccinia virus encoding the prM and E genes of Japanese encephalitis virus (JEV) were purified and characterized. These particles contained the JEV prM/M and E proteins embedded in a lipid bilayer, and RNA was not detected in particles using the polymerase chain reaction and primers recognizing a part of the JEV E gene. The particles were uniformly spherical with a 20-nm diameter and had 5-nm projections on their surface. Mice that received a single inoculation of the purified extracellular particles emulsified with Freund's complete adjuvant were fully protected against 4.9 x 10(5) LD50 of JEV. Comparison of the neutralizing and hemagglutination-inhibiting antibody titers and radioimmunoprecipitation data showed that immunization with the particles induced an immune response similar to that following inoculation with the recombinant vaccinia virus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Viral / genetics
  • Antigens, Viral / immunology*
  • Encephalitis Virus, Japanese / immunology*
  • Encephalitis Virus, Japanese / ultrastructure
  • Genes, Viral
  • HeLa Cells
  • Humans
  • Immunization
  • Mice
  • Protein Precursors / genetics
  • Protein Precursors / immunology
  • Vaccines, Synthetic
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / immunology
  • Viral Proteins / genetics
  • Viral Proteins / immunology*
  • Viral Structural Proteins / genetics
  • Viral Vaccines / immunology


  • Antigens, Viral
  • Protein Precursors
  • Vaccines, Synthetic
  • Viral Envelope Proteins
  • Viral Matrix Proteins
  • Viral Proteins
  • Viral Structural Proteins
  • Viral Vaccines