Subacute sclerosing panencephalitis is typically characterized by alterations in the fusion protein cytoplasmic domain of the persisting measles virus

Virology. 1992 Jun;188(2):910-5. doi: 10.1016/0042-6822(92)90552-z.

Abstract

Our recent extensive analysis of three cases of subacute sclerosing panencephalitis (SSPE) revealed intriguing genetic defects in the persisting measles virus (MV): the fusion (F) genes encoded truncated cytoplasmic F protein domains (Cattaneo et al., Virology 173, 415-425, 1989). Now this MV genomic region has been investigated in eight additional SSPE cases by PCR amplification, replacement cloning into a vector containing the F gene of a lytic MV, in vitro expression, and sequencing. In all cases at least part of the clones showed mutations leading to F protein truncations, elongation, or nonconservative amino acid replacements. It is proposed that alteration of the F protein cytoplasmic domain may play a critical role in the development of SSPE.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cytoplasm
  • Humans
  • Measles virus / genetics*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Sequence Alignment
  • Subacute Sclerosing Panencephalitis / microbiology*
  • Viral Fusion Proteins / genetics*
  • Viral Fusion Proteins / ultrastructure

Substances

  • Oligodeoxyribonucleotides
  • Viral Fusion Proteins

Associated data

  • GENBANK/X16565
  • GENBANK/X16566
  • GENBANK/X16567
  • GENBANK/X16568
  • GENBANK/X16569