Molecular cloning and sequence analysis of the mumps virus gene encoding the L protein and the trailer sequence

Virology. 1992 Jun;188(2):926-30. doi: 10.1016/0042-6822(92)90555-4.

Abstract

We have cloned and determined the nucleotide sequences of the seventh gene of the Miyahara strain of mumps virus (MuV) encoding the L protein. The L gene is 6925 nucleotides in length and contains a single long open reading frame which is capable of coding for a protein of 2261 amino acids with a calculated molecular weight of 256,571 Da. The deduced amino acid sequence of the L protein of MuV showed significant homology with those of six other paramyxoviruses, human parainfluenza type 2 virus, Newcastle disease virus, Sendai virus, measles virus, human parainfluenza type 3 virus, and human respiratory syncytial virus. The predicted MuV L protein contained distinct elements thought to be essential for RNA polymerase activity. A noncoding sequence of 24 nucleotides downstream of the presumed polyadenylation site of the L gene showed significant complementarity with the leader sequence composed of 55 nucleotide at the 3' end of the genomic RNA.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • DNA, Viral / genetics
  • Genes, Viral*
  • Molecular Sequence Data
  • Mumps virus / genetics*
  • RNA Replicase / genetics*
  • Restriction Mapping
  • Viral Structural Proteins / genetics*

Substances

  • DNA, Viral
  • Viral Structural Proteins
  • DNA
  • RNA Replicase

Associated data

  • GENBANK/D10575