Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing

Bioorg Med Chem Lett. 2005 May 16;15(10):2447-51. doi: 10.1016/j.bmcl.2005.03.079.


One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Inteins*
  • Protein Array Analysis*
  • Proteins / chemistry*


  • Proteins