Inhibitory effects of triterpenes isolated from Chuling (Polyporus umbellatus Fries) on free radical-induced lysis of red blood cells

Biol Pharm Bull. 2005 May;28(5):817-21. doi: 10.1248/bpb.28.817.


Chuling, sclerotia of Polyporus umbellatus FRIES, has long been used for urological disorders in traditional medicine. In this study, we demonstrated that Chuling in vitro protects red blood cells from 2,2-azo-bis(2-amidinopropane)dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 50 to 1000 microg/ml. Moreover, tests were carried out to identify the main ingredient of Chuling with scavenging effect on free radicals. Triterpene carboxylic acids isolated from the methanol extract of Chuling, namely, polyporusterone A and polyporusterone B, were found to have inhibitory activities against AAPH-induced lysis of red blood cells. The anti-hemolytic effect was significantly stronger in polyporusterone B compared with polyporusterone A. Furthermore, the ingestion of 150 mg of Chuling was associated with a significant increase in free-radical scavenging effect of plasma in rats.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dose-Response Relationship, Drug
  • Erythrocytes / drug effects*
  • Erythrocytes / metabolism
  • Free Radical Scavengers / isolation & purification
  • Free Radical Scavengers / pharmacology
  • Free Radicals / antagonists & inhibitors*
  • Free Radicals / pharmacology
  • Hemolysis / drug effects*
  • Hemolysis / physiology
  • Humans
  • Male
  • Polyporales* / isolation & purification
  • Triterpenes / isolation & purification
  • Triterpenes / pharmacology*


  • Free Radical Scavengers
  • Free Radicals
  • Triterpenes