Studies on palauan medicinal herbs. II. Activation of mouse macrophages RAW 264.7 by Ongael, leaves of Phaleria cumingii (Meisn.) F. Vill. and its acylglucosylsterols

Biol Pharm Bull. 2005 May;28(5):929-33. doi: 10.1248/bpb.28.929.


The extract of Ongael [leaves of Phaleria cumingii (MEISN.) F. VILL.], a Palauan medicinal herb, enhanced an in vitro phagocytic activity of mouse macrophages RAW 264.7 cells (RAW 264.7). Activity-guided fractionation of the Ongael extract by the in vitro phagocytosis assay using RAW 264.7 led to the isolation of a mixture of acylglucosylsterols (1) as an active constituent along with other inactive constituents, tetracosanol and mangiferin. On the basis of chemical modifications and spectral analyses, the compound 1 was deduced to be a mixture of the known 3-O-(6-O-acyl-beta-D-glucosyl)-beta-sitosterols, the acyl moiety being mainly palmitoyl (57%), oleoyl (12%) and alpha-linolenoyl (12%) with small amount of stearoyl (7%) and linoleoyl (4%).

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cell Line
  • Macrophage Activation / drug effects*
  • Macrophage Activation / physiology
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Mice
  • Phalaris*
  • Phytosterols / chemistry
  • Phytosterols / isolation & purification
  • Phytosterols / pharmacology*
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Plant Leaves
  • Plants, Medicinal* / chemistry


  • Phytosterols
  • Plant Extracts