Early gene response to low-intensity pulsed ultrasound in rat osteoblastic cells

Ultrasound Med Biol. 2005 May;31(5):703-8. doi: 10.1016/j.ultrasmedbio.2005.01.013.


The aim of the current research was to quantify the changes in gene expression in rat bone marrow derived stromal cells (BMSC) to low intensity pulsed ultrasound (LIPUS) during early time points after the ultrasound application. LIPUS at 1.5 MHz, 30 mW/cm(2) was applied to BMSC for a single 20 min treatment. Real-time PCR was carried out to quantify the expression of early response genes and bone differentiation marker genes 0.5, 1, 3, 6 and 12 h after the end of the LIPUS treatment. Compared with the controls, LIPUS treatment resulted in elevated transient expression of early response genes (c-jun, c-myc, COX-2, Egr-1, TSC-22) as well as the bone differentiation marker genes, osteonectin and osteopontin, at 3 h. This induction of early response genes as well as extracellular matrix genes associated with cell proliferation and differentiation may represent the effect of LIPUS to cells of osteoblastic lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells
  • Cells, Cultured
  • Cyclooxygenase 2
  • DNA-Binding Proteins / genetics
  • Early Growth Response Protein 1
  • Gene Expression / genetics*
  • Genes, jun / genetics
  • Genes, myc / genetics
  • Immediate-Early Proteins / genetics
  • Osteoblasts / physiology*
  • Osteonectin / genetics
  • Osteopontin
  • Prostaglandin-Endoperoxide Synthases / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Repressor Proteins / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sialoglycoproteins / genetics
  • Transcription Factors / genetics
  • Ultrasonics*


  • DNA-Binding Proteins
  • Early Growth Response Protein 1
  • Egr1 protein, rat
  • Immediate-Early Proteins
  • Osteonectin
  • Repressor Proteins
  • Sialoglycoproteins
  • Spp1 protein, rat
  • Transcription Factors
  • Tsc22d1 protein, rat
  • Osteopontin
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases