Protein-functionalized polymer brushes

Biomacromolecules. May-Jun 2005;6(3):1602-7. doi: 10.1021/bm050016n.

Abstract

A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O(6)-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O(6)-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Polymers / chemical synthesis*
  • Protein Binding
  • Proteins / chemical synthesis*

Substances

  • Polymers
  • Proteins