A trisialoganglioside containing a sialyl alpha 2-6 N-acetylgalactosamine residue is a cholinergic-specific antigen, Chol-1 alpha

J Biochem. 1992 Mar;111(3):287-90. doi: 10.1093/oxfordjournals.jbchem.a123751.


Cholinergic-specific antigens termed the Chol-1 family have been suggested to be of a ganglioside nature by Richardson et al. (J. Neurochem. 38, 1605-1614, 1982). Two molecular species of polysialogangliosides among bovine brain gangliosides were found to react with anti-Chol-1 alpha antiserum. One of them, Chol-1 alpha-a, was isolated and characterized as a trisialoganglioside containing the gangliotetraose backbone in which 1 mol of sialic acid was attached to each of the reducing end galactose, N-acetylgalactosamine and internal galactose residues, respectively. The chemical structure of Chol-1 alpha-a was determined for the first time, being as follows: IV3NeuAc III6NeuAc II3NeuAc-GgOse4 Cer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Surface / analysis*
  • Cholinergic Fibers / immunology*
  • Chromatography, Thin Layer
  • Gangliosides / analysis*
  • Mass Spectrometry
  • Nerve Endings / immunology


  • Antigens, Surface
  • Gangliosides
  • chol-1 antigen
  • trisialoganglioside