Molecular Cloning and Expression of cDNA Encoding a galactose/N-acetylgalactosamine-specific Lectin on Mouse Tumoricidal Macrophages

J Biochem. 1992 Mar;111(3):331-6. doi: 10.1093/oxfordjournals.jbchem.a123758.

Abstract

We previously reported that the mouse macrophage galacose and N-acetylgalactosamine-specific lectin (MMGL) may participate in the binding of the macrophages to tumor cells [Oda, S., Sato, M., Toyoshima, S., & Osawa, T. (1989) J. Biochem. 105, 1040-1043]. We now report the cloning and characterization of a cDNA encoding MMGL. The MMGL gene encoded a protein consisting of 304 amino acid residues with a molecular weight of 34,595. The deduced amino acid sequence indicated that MMGL had a single membrane-spanning region, three leucine zipper-like domains, and a carbohydrate recognition domain. Two N-glycosylation sites were found in the extracellular region of MMGL, corresponding to the heavy N-glycosylation in the native MMGL. Comparison of the amino acid sequence of MMGL with those of rat hepatic lectins revealed a high overall sequence homology. The sequence homology was especially high in the putative membrane-spanning region and carbohydrate recognition domain. There was, however, a region of 25 amino acids which did not exist on hepatic lectins. The MMGL cDNA without the region encoding the putative membrane-spanning region and intracellular region was expressed in Escherichia coli. The expressed protein had galactose-binding activity and its sugar-binding specificity was same as that of the native lectin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylgalactosamine / pharmacology
  • Amino Acid Sequence
  • Animals
  • Asialoglycoproteins
  • Base Sequence
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • Escherichia coli
  • Female
  • Galactose / pharmacology
  • Gene Expression / genetics
  • Lectins / genetics*
  • Lectins, C-Type*
  • Macrophages / immunology*
  • Membrane Proteins*
  • Mice
  • Mice, Inbred C3H
  • Molecular Sequence Data

Substances

  • Asialoglycoproteins
  • Carrier Proteins
  • Clec10a protein, mouse
  • Clec10a protein, rat
  • Lectins
  • Lectins, C-Type
  • Membrane Proteins
  • Acetylgalactosamine
  • Galactose

Associated data

  • GENBANK/D13975
  • GENBANK/D13976
  • GENBANK/L01141
  • GENBANK/S36676
  • GENBANK/S96732
  • GENBANK/S96733
  • GENBANK/S96735
  • GENBANK/S96741
  • GENBANK/S96751
  • GENBANK/S96754