Isolation of a gene encoding a putative leucine zipper structure that is induced by transforming growth factor beta 1 and other growth factors

J Biol Chem. 1992 May 25;267(15):10219-24.


A gene sequence (TSC-22) that is induced by transforming growth factor (TGF) beta 1 was isolated by differential screening of a lambda gt10 cDNA library constructed from poly(A)+ RNA of mouse osteoblastic cells treated with TGF-beta 1 for 2 h. TSC-22 gene expression was transcriptionally activated by TGF-beta 1. It was also induced by phorbol 12-myristate 13-acetate, serum, cholera toxin, or dexamethasone, but not appreciably by epidermal growth factor. Its induction was rapid and transient, reaching a peak 2 h after TGF-beta 1 treatment, and was resistant to cycloheximide like that of c-jun. The nucleotide sequences of TSC-22 cDNA showed no homology with any known gene sequence. The open reading frame and in vitro translation product indicated that the gene encodes a polypeptide of 143 amino acids with a molecular mass of 18 kDa that contains a putative leucine zipper structure. Polyclonal antibody was raised against TSC-22 protein expressed in Escherichia coli cells, and the antibody detected a 18-kDa protein in both the cytoplasmic and nuclear fractions of [35S]Met-labeled cells. These results indicate that the TSC-22 gene is a new member of the family of early response genes, and encodes a small polypeptide that is a putative transcriptional regulator.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blood
  • Cells, Cultured
  • Cholera Toxin / pharmacology
  • Cycloheximide / pharmacology
  • DNA / genetics
  • Dexamethasone / pharmacology
  • Growth Substances / metabolism
  • Leucine Zippers / genetics*
  • Mice
  • Molecular Sequence Data
  • Osteoblasts / metabolism
  • Precipitin Tests
  • Protein Biosynthesis
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transcription, Genetic
  • Transforming Growth Factor beta / metabolism*


  • Growth Substances
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Dexamethasone
  • DNA
  • Cholera Toxin
  • Cycloheximide
  • Tetradecanoylphorbol Acetate

Associated data

  • GENBANK/D10756
  • GENBANK/D10757
  • GENBANK/L01141
  • GENBANK/S34439
  • GENBANK/S96733
  • GENBANK/S96735
  • GENBANK/S96741
  • GENBANK/S96751
  • GENBANK/S96754
  • GENBANK/X62940