Rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil

Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1133-1137. doi: 10.1099/ijs.0.63390-0.

Abstract

A Gram-positive, aerobic, non-motile, mesophilic strain, designated YIM 70056(T), was isolated from a forest soil sample in Yunnan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this isolate had less than 97.0 % similarity to any Rhodococcus species with validly published names, with the exception of Rhodococcus fascians (DSM 20669(T)), which was found to be its closest neighbour (98.9 % similarity). Chemotaxonomic data, including peptidoglycan type, diagnostic sugar compositions, fatty acid profiles, menaquinones, polar lipids and mycolic acids, were determined for this isolate; the results supported the affiliation of strain YIM 70056(T) to the genus Rhodococcus. The DNA G + C content was 63.5 mol%. The results of DNA-DNA hybridization with R. fascians DSM 20669(T), in combination with chemotaxonomic and physiological data, demonstrated that isolate YIM 70056(T) represents a novel Rhodococcus species, for which the name Rhodococcus yunnanensis sp. nov. is proposed, with YIM 70056(T) (=CCTCC AA 204007(T) = KCTC 19021(T) = DSM 44837(T)) as the type strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Base Composition
  • Carbohydrates / analysis
  • Carbohydrates / isolation & purification
  • China
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / isolation & purification
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / isolation & purification
  • Fatty Acids / analysis
  • Fatty Acids / isolation & purification
  • Genes, rRNA
  • Gentian Violet
  • Molecular Sequence Data
  • Movement
  • Nucleic Acid Hybridization
  • Peptidoglycan / chemistry
  • Peptidoglycan / isolation & purification
  • Phenazines
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Rhodococcus / classification*
  • Rhodococcus / cytology
  • Rhodococcus / isolation & purification*
  • Rhodococcus / physiology
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Temperature
  • Trees
  • Vitamin K 2 / analysis
  • Vitamin K 2 / isolation & purification

Substances

  • Carbohydrates
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Gram's stain
  • Peptidoglycan
  • Phenazines
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Gentian Violet

Associated data

  • GENBANK/AY602219