MSight: an image analysis software for liquid chromatography-mass spectrometry

Proteomics. 2005 Jun;5(9):2381-4. doi: 10.1002/pmic.200401244.


Images obtained from high-throughput mass spectrometry (MS) contain information that remains hidden when looking at a single spectrum at a time. Image processing of liquid chromatography-MS datasets can be extremely useful for quality control, experimental monitoring and knowledge extraction. The importance of imaging in differential analysis of proteomic experiments has already been established through two-dimensional gels and can now be foreseen with MS images. We present MSight, a new software designed to construct and manipulate MS images, as well as to facilitate their analysis and comparison.

MeSH terms

  • Chromatography, Liquid*
  • Electrophoresis, Gel, Two-Dimensional
  • Image Processing, Computer-Assisted*
  • Peptides / analysis
  • Proteome / analysis
  • Software*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*


  • Peptides
  • Proteome