Abstract
To evaluate the potential role of the uPAR/uPA/PAI-1 system in HIV-induced blood-brain-barrier (BBB) disruption, CSF uPA-dependent plasminogen activation (PdPA) was analyzed by casein zymography, and CSF protein levels of all three molecules were measured by ELISA. CSF uPAR, but not uPA, PAI-1, or PdPA levels was significantly increased in neurologically compromised HIV+ patients. Only individual patients with severe AIDS dementia complex had increased levels of uPA (but not PAI-1) which fell upon initiation of antiretroviral therapy. The levels of all three molecules did not correlate with the CSF to serum albumin ratio suggesting not an important role in HIV-induced BBB disruption.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS Dementia Complex / cerebrospinal fluid
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AIDS Dementia Complex / enzymology
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AIDS Dementia Complex / immunology
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CD4 Lymphocyte Count
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HIV Infections / cerebrospinal fluid*
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HIV Infections / enzymology
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HIV Infections / immunology
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HIV Infections / virology
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HIV-1*
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Hemagglutination Inhibition Tests
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Humans
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Plasminogen Activator Inhibitor 1 / cerebrospinal fluid*
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Receptors, Cell Surface / blood
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Receptors, Cell Surface / metabolism*
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Receptors, Urokinase Plasminogen Activator
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Serum Albumin / cerebrospinal fluid
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Solubility
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Urokinase-Type Plasminogen Activator / cerebrospinal fluid*
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Viral Load
Substances
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PLAUR protein, human
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Plasminogen Activator Inhibitor 1
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Receptors, Cell Surface
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Receptors, Urokinase Plasminogen Activator
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Serum Albumin
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Urokinase-Type Plasminogen Activator