Abstract
Much attention has focused on the insulin-like signaling pathway in Caenorhabditis elegans because of its pivotal role in life-span determination and oxidative stress resistance. The daf-16 gene encodes a fork-head transcription factor that is negatively regulated by this insulin-signaling pathway. The DAF-16 protein is translocated to the nucleus when animals were subjected to oxidative stress in the form of paraquat. This oxidative stress-mediated translocation was blocked by mutation of the p38-related sek-1 (MAPKK) mutant and DAF-16 instead remained cytoplasmic. The fact that DAF-16 translocation by oxidative stress is epistatic to sek-1 suggests that oxidative stress mediates regulation of DAF-16 through activating the p38 signal transduction pathway upstream of daf-16 so as to mobilize DAF-16 to the nucleus and activate transcription.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / drug effects
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Active Transport, Cell Nucleus / genetics
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Active Transport, Cell Nucleus / physiology
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Animals
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / physiology*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Cell Nucleus / genetics
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Cell Nucleus / metabolism*
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Forkhead Transcription Factors
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Herbicides / pharmacology
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MAP Kinase Kinase 4 / genetics
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MAP Kinase Kinase 4 / metabolism
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MAP Kinase Signaling System / drug effects
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MAP Kinase Signaling System / genetics
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MAP Kinase Signaling System / physiology*
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Oxidative Stress / drug effects
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Oxidative Stress / genetics
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Oxidative Stress / physiology*
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Paraquat / pharmacology
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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p38 Mitogen-Activated Protein Kinases / metabolism*
Substances
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Caenorhabditis elegans Proteins
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Forkhead Transcription Factors
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Herbicides
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Transcription Factors
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daf-16 protein, C elegans
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p38 Mitogen-Activated Protein Kinases
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MAP Kinase Kinase 4
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Paraquat