Role of oxidized human plasma low density lipoproteins in atherosclerosis: effects on smooth muscle cell proliferation

Mol Cell Biochem. 1992 Apr;111(1-2):143-7. doi: 10.1007/BF00229586.


The effects of oxidized human plasma low density lipoproteins (Ox-LDL) on the proliferation of cultured aortic smooth muscle cells was studied, employing viable cell counting, [3H] thymidine incorporation into DNA, and the release of lactate dehydrogenase (LDH) into the medium. Oxidized LDL (prepared by incubation of LDL with copper sulfate) exerted a concentration-dependent stimulation (2 fold, compared to control) of aortic smooth muscle cell proliferation at low concentrations (0.1 micrograms-10 micrograms/ml medium). On the other hand, at high concentrations (25-200 micrograms/ml), Ox-LDL produced a pronounced decrease in viable cells, a decrease in the incorporation of [3H] thymidine into DNA, and an increase in the release of LDH in the medium. In this report, the previously postulated biological roles of oxidized-LDL in atherosclerosis are discussed in view of these findings.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Arteriosclerosis / blood*
  • Arteriosclerosis / pathology
  • Cell Division
  • Cells, Cultured
  • Humans
  • Lipoproteins, LDL / blood
  • Lipoproteins, LDL / physiology*
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / metabolism*
  • Oxidation-Reduction


  • Lipoproteins, LDL