[A Method for the Preparation of Normalized cDNA Libraries Enriched With Full-Length Sequences]

Bioorg Khim. Mar-Apr 2005;31(2):186-94. doi: 10.1007/s11171-005-0023-7.
[Article in Russian]

Abstract

We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The duplex-specific nuclease is thermostable, it effectively cleaves double-stranded DNA and is inactive toward single-stranded DNA (Shagin et al., Genome Res., 2002, vol. 12, pp. 1935-1942). Our method enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation. The efficiency of the method was demonstrated in model experiments using cDNA samples from several human tissues.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Brachyura
  • DNA, Complementary / chemistry*
  • DNA, Single-Stranded / chemistry*
  • Endonucleases / chemistry
  • Gene Library*
  • Humans
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction

Substances

  • DNA, Complementary
  • DNA, Single-Stranded
  • Endonucleases