Functional analysis of transcriptional repressor Otx3/Dmbx1

FEBS Lett. 2005 May 23;579(13):2926-32. doi: 10.1016/j.febslet.2005.04.042.


Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N2-4)TAATCC] of Otx3/Dmbx1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers
  • Dimerization
  • Homeodomain Proteins / chemistry
  • Homeodomain Proteins / physiology*
  • Mice
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / physiology*
  • Otx Transcription Factors
  • Repressor Proteins / chemistry
  • Repressor Proteins / physiology*
  • Transcription, Genetic / physiology


  • DNA Primers
  • Homeodomain Proteins
  • Nerve Tissue Proteins
  • Otx Transcription Factors
  • Otx3 protein, mouse
  • Repressor Proteins