Enterovirus 70 binds to different glycoconjugates containing alpha2,3-linked sialic acid on different cell lines

J Virol. 2005 Jun;79(11):7087-94. doi: 10.1128/JVI.79.11.7087-7094.2005.

Abstract

Enterovirus 70 (EV70), the causative agent of acute hemorrhagic conjunctivitis, exhibits a restricted tropism for conjunctival and corneal cells in vivo but infects a wide spectrum of mammalian cells in culture. Previously, we demonstrated that human CD55 is a receptor for EV70 on HeLa cells but that EV70 also binds to sialic acid-containing receptors on a variety of other human cell lines. Virus recognition of sialic acid attached to underlying glycans by a particular glycosidic linkage may contribute to host range, tissue tropism, and pathogenesis. Therefore, we tested the possibility that EV70 binds to alpha2,3-linked sialic acid, like other viruses associated with ocular infections. Through the use of linkage-specific sialidases, sialyltransferases, and lectins, we show that EV70 recognizes alpha2,3-linked sialic acid on human corneal epithelial cells and on U-937 cells. Virus attachment to both cell lines is CD55 independent and sensitive to benzyl N-acetyl-alpha-D-galactosaminide, an inhibitor of O-linked glycosylation. Virus binding to corneal cells, but not U-937 cells, is inhibited by proteinase K, but not by phosphatidylinositol-specific phospholipase C treatment. These results are consistent with the idea that a major EV70 receptor on corneal epithelial cells is an O-glycosylated, non-glycosyl phosphatidylinositol-anchored membrane glycoprotein containing alpha2,3-linked sialic acid, while sialylated receptors on U-937 cells are not proteinaceous.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Cell Line
  • Conjunctivitis, Acute Hemorrhagic / etiology
  • Cornea / metabolism
  • Cornea / virology
  • Enterovirus D, Human / pathogenicity*
  • Enterovirus D, Human / physiology*
  • Enterovirus Infections / etiology
  • Epithelial Cells / metabolism
  • Epithelial Cells / virology
  • Glycoconjugates / chemistry
  • Glycoconjugates / metabolism*
  • HeLa Cells
  • Humans
  • Lectins / metabolism
  • Macaca mulatta
  • Neuraminidase / pharmacology
  • Receptors, Virus / chemistry
  • Receptors, Virus / physiology*
  • Sialic Acids / chemistry
  • Sialyltransferases / pharmacology
  • U937 Cells

Substances

  • Glycoconjugates
  • Lectins
  • Receptors, Virus
  • Sialic Acids
  • Sialyltransferases
  • beta-galactoside alpha-2,3-sialyltransferase
  • Neuraminidase