Identification of 26RFa from frog brain: a novel hypothalamic neuropeptide with orexigenic activity in mammals

Ann N Y Acad Sci. 2005 Apr;1040:80-3. doi: 10.1196/annals.1327.009.


In the present study, we report the identification, in the frog brain, of a novel neuropeptide, termed 26RFa, that belongs to the RFamide peptide family. The cDNAs encoding the precursors for 26RFa have been characterized in human and rats. In rats, prepro-26RFa mRNA is expressed exclusively in two hypothalamic nuclei involved in the control of feeding behavior. Intracerebroventricular injection of 26RFa in mice induced a dose-dependent increase in food consumption. Taken together, these data indicate that 26RFa is a novel neuropeptide that may have important biological functions in vertebrates.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anura / genetics*
  • Brain / physiology
  • Humans
  • Hypothalamus / physiology*
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / physiology
  • Molecular Sequence Data
  • Neuropeptides / genetics*
  • Neuropeptides / isolation & purification
  • Neuropeptides / physiology
  • Orexins
  • Rats
  • Sequence Homology, Amino Acid


  • Intracellular Signaling Peptides and Proteins
  • Neuropeptides
  • Orexins
  • RFamide peptide
  • prepro-26RFa protein, human