The transcriptional response to hypoxic insult controlled by FRA-2

Gene Expr. 2005;12(2):61-7. doi: 10.3727/000000005783992160.


FRA-2 is involved in cellular differentiation and is also upregulated in response to ischemic injury to the brain. To shed light on the function of this transcription factor, a novel microarray analysis was utilized to identify FRA-2-dependent gene expression increased in the hypoxic response. Genes were identified that were upregulated by exposure of neuronally differentiated PC12 cells to hypoxia. Using a dominant negative construct to block FRA-2, a second subset of genes that were FRA-2 dependent was found. Cross comparison then allowed isolation of a list of genes that were induced in response to hypoxia in a FRA-2-dependent manner. These data suggest that FRA-2 is involved in the transcriptional control of neuroprotective genes and in the switch from aerobic to anaerobic metabolism.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / metabolism*
  • Blotting, Western
  • Cell Differentiation / drug effects
  • Cell Hypoxia
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Fos-Related Antigen-2
  • Gene Expression Profiling*
  • Genes, Dominant
  • Nerve Growth Factor / pharmacology
  • Oligonucleotide Array Sequence Analysis
  • PC12 Cells / cytology
  • PC12 Cells / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic*
  • Up-Regulation


  • Biomarkers
  • DNA-Binding Proteins
  • Fos-Related Antigen-2
  • Fosl2 protein, rat
  • RNA, Messenger
  • Transcription Factors
  • Nerve Growth Factor