Nectin-like molecule 1 is a protein 4.1N associated protein and recruits protein 4.1N from cytoplasm to the plasma membrane

Biochim Biophys Acta. 2005 May 20;1669(2):142-54. doi: 10.1016/j.bbamem.2005.01.013. Epub 2005 Feb 25.


Nectins are immunoglobulin superfamily adhesion molecules that participate in the organization of epithelial and endothelial junctions. Sharing high homology with the poliovirus receptor (PVR/CD155), nectins were also named poliovirus receptor-related proteins (PRRs). Four nectins and five nectin-like molecules have been identified. Here we describe the cloning and characterization of human and mouse nectin-like molecular 1 (NECL1). Human and mouse NECL1 share 87.3% identity at the amino acid level. NECL1 contains an ectodomain made of three immunoglobulin-like domains, and a cytoplasmic region homologous to those of glycophorin C and contactin-associated protein. RNA blot and in situ hybridization analysis showed that NECL1 predominantly expressed in the central nervous system, mainly in neuronal cell bodies in a variety of brain regions including the cerebellum, cerebral cortex and hippocampus. In vitro binding assay proved the association of NECL1 with protein 4.1N. NECL1 localizes to the cell-cell junctions and recruits protein 4.1N to the plasma membranes through its C-terminus, thus may regulate the function of the cell-cell junction. We propose that the NECL1 and protein 4.1N complex is involved in the morphological development, stability, and dynamic plasticity of the nervous system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blood Proteins / metabolism*
  • Blotting, Western
  • Cell Adhesion Molecule-1
  • Cell Adhesion Molecules
  • Cell Membrane / metabolism*
  • Central Nervous System / embryology
  • Central Nervous System / metabolism
  • Cytoplasm / metabolism*
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / metabolism*
  • Humans
  • Immunoglobulins / genetics
  • Immunoglobulins / metabolism*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • Microtubule-Associated Proteins / metabolism*
  • Molecular Sequence Data
  • Neuropeptides / genetics
  • Neuropeptides / metabolism*
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Tumor Suppressor Proteins


  • Blood Proteins
  • CADM1 protein, human
  • Cadm1 protein, mouse
  • Cell Adhesion Molecule-1
  • Cell Adhesion Molecules
  • Cytoskeletal Proteins
  • Immunoglobulins
  • Membrane Proteins
  • Microtubule-Associated Proteins
  • Neuropeptides
  • RNA, Messenger
  • Tumor Suppressor Proteins
  • erythrocyte membrane band 4.1 protein
  • erythrocyte membrane protein band 4.1-like 1