Abstract
Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Calcium
-
Cerebral Cortex / cytology*
-
Cerebral Cortex / physiology*
-
Fluorescent Dyes
-
Image Processing, Computer-Assisted / methods
-
Imaging, Three-Dimensional / methods*
-
Mice
-
Microscopy, Confocal
-
Neuroglia / cytology*
-
Neurons / cytology*