Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA

Mol Cell Biol. 2005 Jun;25(11):4359-70. doi: 10.1128/MCB.25.11.4359-4370.2005.


N(2)-Monomethylguanosine-10 (m(2)G10) and N(2),N(2)-dimethylguanosine-26 (m(2)(2)G26) are the only two guanosine modifications that have been detected in tRNA from nearly all archaea and eukaryotes but not in bacteria. In Saccharomyces cerevisiae, formation of m(2)(2)G26 is catalyzed by Trm1p, and we report here the identification of the enzymatic activity that catalyzes the formation of m(2)G10 in yeast tRNA. It is composed of at least two subunits that are associated in vivo: Trm11p (Yol124c), which is the catalytic subunit, and Trm112p (Ynr046w), a putative zinc-binding protein. While deletion of TRM11 has no detectable phenotype under laboratory conditions, deletion of TRM112 leads to a severe growth defect, suggesting that it has additional functions in the cell. Indeed, Trm112p is associated with at least four proteins: two tRNA methyltransferases (Trm9p and Trm11p), one putative protein methyltransferase (Mtc6p/Ydr140w), and one protein with a Rossmann fold dehydrogenase domain (Lys9p/Ynr050c). In addition, TRM11 interacts genetically with TRM1, thus suggesting that the absence of m(2)G10 and m(2)(2)G26 affects tRNA metabolism or functioning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Computational Biology
  • Guanosine / analogs & derivatives*
  • Guanosine / metabolism*
  • Methylation
  • Methyltransferases / genetics
  • Methyltransferases / metabolism
  • Methyltransferases / physiology*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • RNA, Transfer / metabolism*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / physiology*
  • Sequence Deletion
  • Sequence Homology, Amino Acid
  • tRNA Methyltransferases / genetics
  • tRNA Methyltransferases / metabolism
  • tRNA Methyltransferases / physiology*


  • Saccharomyces cerevisiae Proteins
  • Guanosine
  • 7-methylguanosine
  • RNA, Transfer
  • Methyltransferases
  • tRNA Methyltransferases
  • Trm11 protein, S cerevisiae
  • Trm112 protein, S cerevisiae