Polymorphism of Aeromonas spp. tRNA intergenic spacers

Syst Appl Microbiol. 2005 Apr;28(3):222-9. doi: 10.1016/j.syapm.2004.12.008.

Abstract

We investigated the length polymorphism of the intergenic spacers lying between tRNA genes of Aeromonas spp. A total of 69 strains representing all known genomic species of Aeromonas were used in the study. tDNA-PCR patterns were examined by Dice coefficient (S(D)) and unweighted pair group method of clustering (UPGMA). The strains were allocated into 15 groups at a similarity level of 70%. The strains belonging to seven genomic species: A. hydrophila (HG 1), A. caviae (HG 4), A. sobria (HG 7), A. veronii (HG 8/10), A. encheleia (HG 16), A. popoffii (HG 17), and A. culicicola (HG 18) formed distinct clusters. Our study revealed a genetic heterogeneity of the following species: A. bestiarum, A. salmonicida, A. media, A. eucrenophila, A. jandaei, A. schubertii, and A. allosaccharophila.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas / classification*
  • Aeromonas / genetics*
  • Aeromonas / isolation & purification
  • Animals
  • Cluster Analysis
  • DNA Fingerprinting
  • DNA, Bacterial / genetics
  • DNA, Intergenic / genetics*
  • Food Microbiology
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • RNA, Bacterial / genetics
  • RNA, Transfer / genetics*
  • Water Microbiology

Substances

  • DNA, Bacterial
  • DNA, Intergenic
  • RNA, Bacterial
  • RNA, Transfer