The eefABC multidrug efflux pump operon is repressed by H-NS in Enterobacter aerogenes

J Bacteriol. 2005 Jun;187(11):3894-7. doi: 10.1128/JB.187.11.3894-3897.2005.


The Enterobacter aerogenes eefABC locus, which encodes a tripartite efflux pump, was cloned by complementation of an Escherichia coli tolC mutant. E. aerogenes deltaacrA expressing EefABC became less susceptible to a wide range of antibiotics. Data from eef::lacZ fusions showed that eefABC was not transcribed in the various laboratory conditions tested. However, increased transcription from Peef was observed in an E. coli hns mutant. In addition, EefA was detected in E. aerogenes expressing a dominant negative E. coli hns allele.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Proteins / genetics*
  • DNA-Binding Proteins / genetics*
  • Enterobacter aerogenes / genetics*
  • Escherichia coli / genetics
  • Genes, MDR / genetics*
  • Genetic Complementation Test
  • Lac Operon
  • Molecular Sequence Data
  • Mutation
  • Operon / physiology*
  • Suppression, Genetic


  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • DNA-Binding Proteins
  • H-NS protein, bacteria

Associated data

  • GENBANK/AJ508047