The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes

J Immunol. 2005 Jun 1;174(11):6967-73. doi: 10.4049/jimmunol.174.11.6967.

Abstract

The antiapoptotic protein Bcl-x(L) is induced in activated T lymphocytes upon costimulation through CD28, 4-1BB, and OX40. Bcl-x(L) is also highly enriched in memory T lymphocytes. Based on this body of evidence, it was thought that Bcl-x(L) plays an essential role in the generation of effector and memory T lymphocytes. We report that mice with a conditional deletion of Bcl-x in T lymphocytes develop a normal CD8(+) T cell response to Listeria monocytogenes infection. Furthermore, Bcl-x conditional knockout mice exhibit normal T-dependent humoral immune responses. These results indicate that Bcl-x is dispensable for the generation of effector and memory T lymphocytes and suggest that costimulation of T lymphocytes promotes their survival through a Bcl-x(L) independent mechanism.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apoptosis / genetics
  • Apoptosis / immunology*
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology*
  • Cell Survival / genetics
  • Cell Survival / immunology
  • Cytotoxicity, Immunologic* / genetics
  • Female
  • Immunization, Secondary
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin M / biosynthesis
  • Immunologic Memory* / genetics
  • Listeriosis / genetics
  • Listeriosis / immunology
  • Listeriosis / pathology
  • Lymphocyte Activation / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Proto-Oncogene Proteins c-bcl-2 / deficiency
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Proto-Oncogene Proteins c-bcl-2 / physiology*
  • T-Lymphocyte Subsets / cytology
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / metabolism
  • T-Lymphocytes, Cytotoxic / cytology
  • T-Lymphocytes, Cytotoxic / immunology
  • T-Lymphocytes, Cytotoxic / metabolism
  • T-Lymphocytes, Helper-Inducer / cytology
  • T-Lymphocytes, Helper-Inducer / immunology
  • T-Lymphocytes, Helper-Inducer / metabolism
  • bcl-X Protein

Substances

  • Bcl2l1 protein, mouse
  • Immunoglobulin G
  • Immunoglobulin M
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-X Protein