Systematic screening of nuclear encoded proteins involved in the splicing metabolism of group II introns in yeast mitochondria

Gene. 2005 Jul 18;354:72-9. doi: 10.1016/j.gene.2005.03.023.

Abstract

Studies of yeast, algae and plants have provided genetic and biochemical evidence that the splicing reaction of organellar localized group II introns either depends on proteins encoded by the introns themselves ('maturases') or encoded by other genes of the host organisms. However, only a few of those proteins have been identified to date and characterized in more detail. In order to find new nuclear encoded proteins that assist group II splicing, we screened a complete knockout library of Saccharomyces cerevisiae strain BY4741 consisting of 4878 viable haploid clones. The strain contains a rho+ mitochondrial genome with a set of 13 introns including the three group II introns (aI1, aI2, aI5gamma) in the gene encoding cytochrome-c-oxidase subunit 1 (COX1) and the single group II intron (bI1) in the gene encoding cytochrome b (CYTB). In our screen and initial molecular analysis, we focus on intron aI5gamma, the last intron in the COX1 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / genetics
  • DNA, Mitochondrial / genetics*
  • Electron Transport Chain Complex Proteins / genetics
  • Electron Transport Complex IV / genetics
  • Gene Library
  • Genetic Complementation Test
  • Introns / genetics*
  • Mitochondrial Proteins / genetics*
  • Mitochondrial Proteins / isolation & purification
  • Mutation
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / isolation & purification
  • Phenotype
  • RNA Splicing*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / isolation & purification

Substances

  • DNA, Mitochondrial
  • Electron Transport Chain Complex Proteins
  • Mitochondrial Proteins
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins
  • Electron Transport Complex IV