Expression of 14-3-3 zeta and interaction with protein kinase C in the rat retina in early diabetes

Diabetologia. 2005 Jul;48(7):1411-5. doi: 10.1007/s00125-005-1774-7. Epub 2005 May 21.


Aims/hypothesis: The present study aimed to investigate the expression levels of and the relationship between 14-3-3 zeta and protein kinase C (PKC) in the retina of early diabetes.

Methods: Changes in the expression levels of, and interaction between, 14-3-3 zeta and PKC were investigated by Northern and Western blot analyses, immunoprecipitation and double immunostaining in the retina of diabetic rats after 6 weeks of diabetes. PKC activity was examined using a PKC assay.

Results: In the diabetic retina, the molecular levels of 14-3-3 zeta were reduced, while those of PKC beta and zeta were increased. Direct interaction between 14-3-3 zeta and PKC was markedly decreased in the retina after 6 weeks of diabetes, while PKC activity was increased.

Conclusions/interpretation: These findings show that a reduction in 14-3-3 zeta can induce PKC activation, suggesting that this is a main cause of visual dysfunction in the retina during diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / genetics*
  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Diabetes Mellitus, Experimental / enzymology
  • Diabetes Mellitus, Experimental / physiopathology*
  • Gene Expression Regulation
  • Male
  • Protein Kinase C / genetics*
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Retina / physiopathology*
  • Transcription, Genetic


  • 14-3-3 Proteins
  • RNA, Messenger
  • Protein Kinase C