Utilising retention correlation for the separation of oligostyrenes by coupled-column liquid chromatography

Michael J Gray; Gary R Dennis; Patrick J Slonecker; R Andrew Shalliker

doi:10.1016/j.chroma.2004.10.071

Utilising retention correlation for the separation of oligostyrenes by coupled-column liquid chromatography

J Chromatogr A. 2005 May 6;1073(1-2):3-9. doi: 10.1016/j.chroma.2004.10.071.

Authors

Michael J Gray¹, Gary R Dennis, Patrick J Slonecker, R Andrew Shalliker

Affiliation

¹ Nanoscale Organization and Dynamic Group, University of Western Sydney, Hawkesbury, Parramatta, NSW 1797, Australia.

PMID: 15909499
DOI: 10.1016/j.chroma.2004.10.071

Abstract

The separation of n = 2-5 n-butyloligostyrenes has been illustrated by reversed-phase reversed-phase (RP-RP) coupled-column liquid chromatography. The coupled-column separation has been achieved by use of a C18 column with methanol as the mobile phase followed by a DiamondBond C18 column with acetonitrile (ACN) mobile phase. The DiamondBond C18 is a hybrid carbon clad zirconia (CCZ)-C18 stationary phase. Unlike a C18-carbon clad zirconia two-dimensional liquid chromatographic system, which is orthogonal, the C18 and DiamondBond C18 columns combination exhibit correlations based upon the molecular weight of n-butyloligostyrenes. Using an alternative strategy to two-dimensional liquid chromatography, the molecular weight dependence displayed by both the C18 column and DiamondBond C18 has been used to increase throughput or decrease analysis time in the analysis of the n-butyloligostyrenes. However, this is at the expense of a portion of the two-dimensional peak capacity displayed by the C18-carbon clad zirconia system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid / methods*
Molecular Weight
Polymers / isolation & purification*
Styrenes / isolation & purification*

Substances

Polymers
Styrenes