Extraction and chromatography of carotenoids from pumpkin

J Chromatogr A. 2005 May 6;1073(1-2):371-5. doi: 10.1016/j.chroma.2004.10.044.


Vitamin A deficiency is a health problem in Southeast Asia that can be corrected by feeding orange fruits and vegetables such as mango. Pumpkin is a traditional Korean food that is easy to store and is already believed to have health benefits. We extracted carotenoids from pumpkin by liquid-liquid extraction and by supercritical fluid extraction. We measured carotenoids by reversed-phase chromatography with diode array detection. The major carotenoid in pumpkin (> 80%) is beta-carotene, with lesser amounts of lutein, lycopene, alpha-carotene and cis-beta-carotene. Pumpkin is a rich source of beta-carotene and might be useful for preventing Vitamin A deficiency.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Carotenoids / analysis
  • Carotenoids / isolation & purification*
  • Chromatography, Supercritical Fluid / methods*
  • Cucurbita / chemistry*


  • Carotenoids