Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays

EMBO J. 1985 Jan;4(1):17-23.

Abstract

A newly isolated, unstable mutation wx-844::En-1 of Zea mays was proven to be caused by the insertion of the autonomous transposable element En into the Waxy (Wx) gene. Molecular analysis revealed that En-1 is 8.4 kb long, has a 13-bp long perfect inverted repeat at its termini and generates a 3-bp target site duplication. En-1 is integrated into an intron located approximately in the middle of the transcribed region of the Wx gene. Structural evidence is presented indicating that a receptor component (Inhibitor) can arise by internal deletion of an autonomous En element.

MeSH terms

  • Alleles
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Transposable Elements / genetics*
  • Gene Deletion
  • Genes, Plant / genetics
  • Introns / genetics
  • Molecular Sequence Data
  • Phenotype
  • Zea mays / genetics*

Substances

  • DNA Transposable Elements