c-abl has a sequence-specific enhancer binding activity

Cell. 1992 May 29;69(5):751-7. doi: 10.1016/0092-8674(92)90287-m.

Abstract

The enhancers of several distinct viruses contain a common functional element, termed EP. This element binds ubiquitous cellular proteins and generates specific complexes in gel retardation analysis. Ultraviolet cross-linking and Southwestern analysis showed that a 140 kd polypeptide is the major EP DNA-binding protein. Using a combination of DNA binding and immunological techniques, we have identified the c-abl protein in a nuclear complex that binds to the EP element. abl was found to have both a specific and high affinity DNA binding activity. The ability to bind DNA is abolished in the mutant abl protein, p210bcr-abl, consistent with its cytoplasmic localization in chronic myelogenous leukemia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • DNA Probes / genetics
  • DNA, Viral / metabolism*
  • Enhancer Elements, Genetic / genetics*
  • Genes, abl
  • HeLa Cells
  • Hepatitis B virus / genetics
  • Humans
  • Molecular Sequence Data
  • Precipitin Tests
  • Proto-Oncogene Proteins c-abl / genetics
  • Proto-Oncogene Proteins c-abl / metabolism*

Substances

  • DNA Probes
  • DNA, Viral
  • Proto-Oncogene Proteins c-abl