A critical role of lambda 5 protein in B cell development

Cell. 1992 May 29;69(5):823-31. doi: 10.1016/0092-8674(92)90293-l.


The lambda 5 gene is a homolog of immunoglobulin J lambda-C lambda genes, expressed specifically in immature B-lineage cells. Lambda 5-encoded molecules form membrane complexes with mu or D mu proteins in association with an additional protein specifically expressed in immature B cells that is encoded by the Vpre-B gene. We have generated mice in which the lambda 5 gene is inactivated by targeted gene disruption in embryonic stem cells. In these mice, B cell development in the bone marrow is blocked at the pre-B cell stage. However, the blockade is leaky, allowing B cells to populate the peripheral immune system at a low rate. These cells are allelically excluded and able to respond to antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / immunology
  • Base Sequence
  • Cell Differentiation / genetics
  • Female
  • Genes, Immunoglobulin / genetics*
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Immunoglobulin Constant Regions / genetics*
  • Immunoglobulin Joining Region / genetics*
  • Immunoglobulin Variable Region / genetics
  • Immunoglobulin lambda-Chains / genetics*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Mutation / genetics
  • Neomycin / pharmacology
  • Polymerase Chain Reaction
  • Recombination, Genetic / genetics


  • Immunoglobulin Constant Regions
  • Immunoglobulin Joining Region
  • Immunoglobulin Variable Region
  • Immunoglobulin lambda-Chains
  • Neomycin