Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus

J Virol. 2005 Jun;79(12):7738-44. doi: 10.1128/JVI.79.12.7738-7744.2005.


In vitro infection of bovine cells of many origins with the cytopathogenic bovine viral diarrhea virus (cpBVDV) results in the induction of alpha/beta interferon (IFN-alpha/beta), whereas noncytopathogenic BVDV (ncpBVDV) isolates have been shown not to induce IFN-alpha/beta in vitro. Similarly, cpBVDV induces IFN-alpha/beta in the early bovine fetus, but ncpBVDV does not. However, acute infection of naive cattle with ncpBVDV results in IFN-alpha/beta production. In this study, we identified and characterized a minor population of cells, present in lymph nodes that produce IFN-alpha in response to ncpBVDV. These cells expressed the myeloid markers CD14, CD11b, and CD172a but did not express CD4 and CD45RB. We also established that these cells produced IFN-alpha in the absence of detectable productive infection.

MeSH terms

  • Animals
  • Antigens, CD
  • Bovine Virus Diarrhea-Mucosal Disease / immunology*
  • Bovine Virus Diarrhea-Mucosal Disease / virology
  • CD11b Antigen / metabolism
  • Cattle
  • Cytopathogenic Effect, Viral*
  • Diarrhea Viruses, Bovine Viral / immunology*
  • Diarrhea Viruses, Bovine Viral / pathogenicity
  • Interferon-alpha / metabolism*
  • Interferon-beta / metabolism*
  • Lipopolysaccharide Receptors / metabolism
  • Lymph Nodes / cytology*
  • Lymph Nodes / immunology
  • Membrane Glycoproteins / metabolism
  • Neural Cell Adhesion Molecules / metabolism


  • Antigens, CD
  • CD11b Antigen
  • Interferon-alpha
  • Lipopolysaccharide Receptors
  • Membrane Glycoproteins
  • Neural Cell Adhesion Molecules
  • Interferon-beta