Performance of an internal-loop airlift bioreactor for treatment of hexane-contaminated air

Appl Biochem Biotechnol. 2005 Spring:121-124:581-91. doi: 10.1385/abab:122:1-3:0581.

Abstract

Hexane is a toxic volatile organic compound that is quite abundant in gas emissions from chemical industries and printing press and painting centers, and it is necessary to treat these airstreams before they discharge into the atmosphere. This article presents a treatment for hexane-contaminated air in steady-state conditions using an internal-loop airlift bioreactor inoculated with a Pseudomonas aeruginosa strain. Bioprocesses were conducted at 20-mL/min, a load of 1.26 gm3 of C6H14, and a temperature of 28 degrees C. The results of hexane removal efficiencies were presented as a function of the inoculum size (approx 0.07 and 0.2 g/L) and cell reuse. Bioprocess monitoring comprises quantification of the biomass, the surface tension of the medium, and the hexane concentration in the fermentation medium as well as in the inlet and outlet airstreams. The steady-state results suggest that the variation in inoculum size from 0.07 to 0.2 g/L promotes hexane abatement from the influent from 65 to 85%, respectively. Total hydrocarbon removal from the waste gas was achieved during experiments conducted using reused cells at an initial microbial concentration of 0.2 g/L.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Air
  • Air Pollutants / isolation & purification
  • Air Pollutants / pharmacokinetics*
  • Air Pollution / prevention & control
  • Biodegradation, Environmental
  • Bioreactors / classification
  • Bioreactors / microbiology*
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods
  • Cell Proliferation
  • Equipment Design
  • Equipment Failure Analysis
  • Hexanes / isolation & purification*
  • Hexanes / pharmacokinetics*
  • Pseudomonas aeruginosa / growth & development
  • Pseudomonas aeruginosa / metabolism*

Substances

  • Air Pollutants
  • Hexanes