Expression of PKC-beta or cyclin D2 predicts for inferior survival in diffuse large B-cell lymphoma

Mod Pathol. 2005 Oct;18(10):1377-84. doi: 10.1038/modpathol.3800434.


We sought to determine whether identification of poor-risk subgroups of diffuse large B-cell lymphoma (DLBCL) using immunohistochemical stains would have practical utility with regard to prognosis and therapeutic decisions. Tissue microarray blocks were created using replicate samples of formalin-fixed, paraffin-embedded tissue from 200 cases of de novo DLBCL. The sections were stained with antibodies to proteins that are expressed by activated or proliferating B cells including MUM1, FOXP1, bcl-2, survivin, protein kinase C-beta (PKC-beta), cyclin D2, cyclin D3, and Ki-67. In univariate analysis, tumor expression of cyclin D2 (P = 0.025) or PKC-beta (P = 0.015) was associated with a worse overall survival, whereas none of the other markers was predictive of overall survival. Patients with DLBCL that expressed either cyclin D2 or PKC-beta had a 5-year overall survival of only 30% as compared to 52% for those who were negative for both markers (P = 0.0019). In multivariate analysis, the expression of cyclin D2 or PKC-beta was an independent predictor of poor overall survival (P = 0.035). Cyclin D2 and PKC-beta expression will be useful in designing a 'biological prognostic index' for patients with DLBCL.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / metabolism
  • Cyclin D2
  • Cyclins / biosynthesis*
  • Humans
  • Lymphoma, B-Cell / metabolism
  • Lymphoma, B-Cell / mortality*
  • Middle Aged
  • Predictive Value of Tests
  • Protein Kinase C / biosynthesis*
  • Protein Kinase C beta
  • Survival Rate
  • Tissue Array Analysis


  • Biomarkers, Tumor
  • CCND2 protein, human
  • Cyclin D2
  • Cyclins
  • Protein Kinase C
  • Protein Kinase C beta