Abstract
To investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of growth hormone (GH) linked to enhanced yellow fluorescein protein (EYFP). This stable GH3 cell secretes secretory granules of GH linked to EYFP on stimulation by Ca2+ influx or Ca2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH.
MeSH terms
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Animals
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Bacterial Proteins / genetics*
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Biological Transport
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Calcium / metabolism
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Cell Line / metabolism*
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Growth Hormone / genetics
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Growth Hormone / metabolism*
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Luminescent Proteins / genetics*
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Pituitary Gland / cytology*
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Pituitary Gland / metabolism
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Rats
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism*
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Transfection
Substances
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Bacterial Proteins
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Luminescent Proteins
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Recombinant Fusion Proteins
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yellow fluorescent protein, Bacteria
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Growth Hormone
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Calcium