Stable T cell-dendritic cell interactions precede the development of both tolerance and immunity in vivo

Nat Immunol. 2005 Jul;6(7):707-14. doi: 10.1038/ni1210. Epub 2005 May 29.


The maturation status of dendritic cells (DCs) determines whether they prime or tolerize T cells. We targeted ovalbumin peptide exclusively to DCs in situ using an antibody to DEC-205 and studied the interaction of DCs with naive CD4(+) T cells in tolerizing or priming conditions. We used two-photon microscopy to simultaneously track antigen-specific OT-II T cells, nonspecific T cells and DCs in lymph nodes of living mice. In both tolerance and immunity, OT-II cells arrested on DCs near high endothelial venules beginning shortly after extravasation and regained their baseline speed by 18 h. Thus, early antigen-dependent T cell arrest on DCs is a shared feature of tolerance and priming associated with activation and proliferation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD / immunology
  • Bacterial Proteins / chemistry
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Communication / immunology*
  • Cell Movement / immunology
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Immune Tolerance / immunology*
  • Lectins, C-Type / immunology
  • Luminescent Proteins / chemistry
  • Lymph Nodes / cytology
  • Lymph Nodes / immunology
  • Lymphocyte Activation / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Confocal
  • Minor Histocompatibility Antigens
  • Ovalbumin / immunology
  • Receptors, Cell Surface / immunology
  • Specific Pathogen-Free Organisms


  • Antigens, CD
  • Bacterial Proteins
  • DEC-205 receptor
  • Lectins, C-Type
  • Luminescent Proteins
  • Minor Histocompatibility Antigens
  • Receptors, Cell Surface
  • yellow fluorescent protein, Bacteria
  • Ovalbumin