Molecular interactions between the components of the En-I transposable element system of Zea mays

EMBO J. 1985 Mar;4(3):579-83.

Abstract

The sequence of the Inhibitor element Spm-I8 isolated from the wx-m8 allele has been determined. The element is 2242 bp in length. Its ends can be folded into long stem and loop structures. In a line containing the autonomous En element (wx-m8+En) we have detected a 2.5-kb transcript hybridizing to Spm-I8. A cDNA copy of this En-specific transcript containing 1.2 kb of the 3' end was cloned and it DNA sequence was determined. The 3' half of the cDNA is homologous to Spm-I8 and the region of homology is interrupted by intervening sequences. In the absence of an autonomous En element two chimeric transcripts are observed in the wx-m8 line which are probably initiated at the wx promoter and terminate in the Spm-I8 insertion. In the presence of the En element, these transcripts are suppressed, possibly by a trans-acting function of En, inhibiting transcription read-through into Spm-I8.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • DNA Transposable Elements / genetics*
  • DNA, Complementary / genetics
  • DNA, Plant / genetics
  • Genes, Plant / genetics
  • Molecular Sequence Data
  • Zea mays / genetics*

Substances

  • DNA Transposable Elements
  • DNA, Complementary
  • DNA, Plant