Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans

Biotechnol Lett. 2005 Apr;27(7):465-70. doi: 10.1007/s10529-005-2222-y.

Abstract

An actinomycetes expression vector (pIBR25) was constructed and applied to express a gene from the kanamycin biosynthetic gene cluster encoding 2-deoxy-scyllo-inosose synthase (kanA) in Streptomyces lividans TK24. The expression of kanA in pIBR25 transformants reached a maximum after 72 h of culture. The plasmid pIBR25 showed better expression than pSET152, and resulted in the formation of insoluble KanA when it was expressed in Escherichia coli. This strategy thus provides a valuable tool for expressing aminoglycoside-aminocyclitols (AmAcs) biosynthetic genes in Streptomyces spp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression
  • Genetic Vectors*
  • Kanamycin / biosynthesis
  • Lyases / biosynthesis*
  • Lyases / genetics*
  • Streptomyces lividans / enzymology*
  • Streptomyces lividans / genetics*

Substances

  • Kanamycin
  • Lyases
  • 2-deoxy-scyllo-inosose synthase