Abstract
Production of the artificial sweetener, lactosucrose, by various microorganisms containing levansucrase activity was investigated. Of the tested bacteria, Bacillus subtilis was the most effective producer using lactose as an acceptor and sucrose as a fructosyl donor. Lactosucrose production by this strain was optimal at pH 6.0 and 55 degrees C whereupon 181 g lactosucrose l(-1) was produced from 225 g lactose l(-1) and 225 g sucrose l(-1) in 10 h.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / enzymology
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Bacillus subtilis / metabolism
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Bacteria / enzymology*
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Bacteria / metabolism*
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Disaccharides / biosynthesis*
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Hexosyltransferases / metabolism*
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Hydrogen-Ion Concentration
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Lactose / analogs & derivatives
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Lactose / metabolism*
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Sucrose / analogs & derivatives
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Sucrose / metabolism*
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Sweetening Agents / metabolism*
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Temperature
Substances
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Disaccharides
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Sweetening Agents
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Sucrose
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Hexosyltransferases
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levansucrase
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Lactose