Daily intake of multivitamins during long-term intake of olestra in men prevents declines in serum vitamins A and E but not carotenoids

J Nutr. 2005 Jun;135(6):1456-61. doi: 10.1093/jn/135.6.1456.


The objective of this study was to determine whether vitamin supplementation during long-term (36 wk) ingestion of olestra supplemented with vitamin E could prevent decreases in vitamin E, vitamin A, and carotenoids. This was a 36-wk study of 37 healthy males randomly assigned to consume a control diet composed of 33% energy from fat, a similar diet in which one third of the energy from fat had been replaced with olestra, or a fat-reduced (25% of energy from fat) diet. Subjects also ingested a daily multivitamin (Centrum). Serum concentrations of alpha-tocopherol, retinol, beta-carotene, lycopene, and lutein + zeaxanthin were analyzed by HPLC. Subjects eating the olestra-containing diet had substantial decreases in serum beta-carotene, lycopene, and lutein + zeaxanthin, which occurred by 12 wk; these changes were found despite correcting for serum total cholesterol or BMI. Serum beta-carotene and lycopene concentrations were below the lower limit of the reference range (<0.186 and <0.298 mumol/L, respectively) at one or more time points. The slight decline in serum alpha-tocopherol concentration, significant at 24 wk, was caused by the decline in serum cholesterol. Retinol concentrations decreased with time in all 3 groups, but were not affected by olestra. We conclude that supplementation with a multivitamin containing vitamins A and E was adequate to prevent olestra-induced decrease in serum alpha-tocopherol and retinol. Olestra-induced decreases in serum beta-carotene, lycopene, and lutein + zeaxanthin were not prevented by the vitamin supplement used in this study.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Carotenoids / blood*
  • Diet, Fat-Restricted
  • Dietary Fats, Unsaturated / administration & dosage*
  • Dietary Fats, Unsaturated / adverse effects
  • Dietary Fats, Unsaturated / pharmacology
  • Drug Administration Schedule
  • Drug Combinations
  • Fat Substitutes / administration & dosage*
  • Fat Substitutes / adverse effects
  • Fat Substitutes / pharmacology
  • Fatty Acids / administration & dosage*
  • Fatty Acids / adverse effects
  • Fatty Acids / pharmacology
  • Humans
  • Male
  • Middle Aged
  • Sucrose / administration & dosage*
  • Sucrose / adverse effects
  • Sucrose / analogs & derivatives*
  • Sucrose / pharmacology
  • Vitamin A / blood*
  • Vitamin E / blood*
  • Vitamins / administration & dosage*
  • Vitamins / pharmacology


  • Dietary Fats, Unsaturated
  • Drug Combinations
  • Fat Substitutes
  • Fatty Acids
  • Vitamins
  • Vitamin A
  • Vitamin E
  • Carotenoids
  • Sucrose
  • sucrose polyester